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. Author manuscript; available in PMC: 2020 Jul 6.
Published in final edited form as: Cell Rep. 2020 Jun 2;31(9):107704. doi: 10.1016/j.celrep.2020.107704

KEY RESOURCES TABLE

REAGENT or RESOCURCE SOURCE IDENTIFIER
Antibodies
Mouse monoclonal anti-Tubulin Sigma Cat#: T9026; RRID AB_477593
Mouse monoclonal anti-FLAG Sigma Cat#: F1804; RRID: AB_262044
Rat monoclonal anti-HA Roche Cat#: 11867423001; RRID:AB_390918
Rabbit polyclonal anti-P2X3 Alomone Cat#: APR-016; RRID:AB_2313760
Mouse monoclonal anti-NR1 BD Biosciences Cat#: 556308; RRID: AB_396353
Chemicals, Peptides, and Recombinant Proteins
Halt protease inhibitor cocktail ThermoFisher Scientific Cat#: 78446
GlutaMax GIBCO 35050–061
L-Glutamine GIBCO 25030–081
Penicillin Streptomycin GIBCO 15140–122
poly-D-lysine Sigma Cat#: P6407
Protein A Sepharose CL4B GE Healthcare Cat#: 17-0780-01
Sulfo-NHS-SS-Biotin Thermo Scientific Cat#: 21331
NeutraAvidin Plus Ultralink Resin Thermo Scientific Cat#: 53151
FuGENE® 6 Transfection Reagent Promega Cat#: E2691
Trypsin-EDTA (0.05%), phenol red GIBCO Cat#: 25300054
Advanced DMEM GIBCO Cat#: 12491023
Adenosine 5′-triphosphate magnesium salt Sigma Cat#: A9187
TNP-ATP triethylammonium salt Tocris Cat#: 2464
α, β-Methyleneadenosine 5′-triphosphate lithium salt Sigma Cat#: M6517
Ham’s F-12 Nutrient Mix GIBCO Cat#: 11765054
NGF 2.5S Native Mouse Protein GIBCO Cat#: 13257019
Collagenase P Roche Cat#: 11213857001
Fetal Bovine Serum, qualified, USDA-approved regions GIBCO Cat#: 10437028
Trypsin-EDTA (0.25%), phenol red GIBCO Cat#: 25200056
L-Glutamic acid monosodium salt hydrate Sigma Cat#: G5889
(−)-(α)-Kainic Acid (hydrate) Cayman Chemical Company Cat#: 78050
Acetylcholine chloride Sigma Cat#: A5625
Cyclothiazide Sigma Cat#: C9847
HBSS, no calcium, no magnesium, no phenol red GIBCO Cat#: 14175-095
MEM Non-Essential Amino Acids Solution (100X) GIBCO Cat#: 11140050
Gentamycin GIBCO Cat#: 11811-031
Sodium Pyruvate Sigma Cat#: P5280-25G
Hygromycin B (50 mg/mL) GIBCO Cat#: 10687010
Gateway LR Clonase II Enzyme mix Invitrogen Cat#: 11791-020
Minimum Essential Medium GIBCO Cat#: 11090-081
Collagenase from Clostridium histolyticum Sigma Cat#: C9891-1G
Trypsin from bovine pancreas Sigma Cat#: T1426-1G
Deoxyribonuclease I from bovine pancreas Sigma Cat#: D4527
Cytosine β-D-arabinofuranoside hydrochloride Sigma Cat#: C6645-25MG
Insulin-transferrin-sodium selenite media supplement Sigma Cat#: I1884-1VL
2-Mercaptoethanol Sigma Cat#: M3148-25ML
Dithiothreitol American Bioanalytical Cat#: AB00490-00010
Basal Eagle’s medium GIBCO Cat#: 21010-046
Geneticin GIBCO Cat#: 15710-064
Critical Commercial Assays
mMESSAGE mMACHINE T7 Transcription Kit Ambion Cat#: AM1344
Pierce BCA Protein Assay Kit Pierce Cat#: 23225
5Prime Perfectprep® Plasmid 96 VAC Base Kit 5Prime Cat#: 2300320
SuperSignal ELISA Femto Maximum Sensitivity Substrate Thermo Scientific Cat#: 37074
Experimental Models: Cell Lines
HEK293 ATCC Cat#: CRL-1573
293FT Life Technologies Cat#: R70007
Experimental Models: Organisms/Strains
Mouse: Wild-type (C57BL/6J) The Jackson Laboratory Stock#: 000664
Recombinant DNA
Plasmid: pGEM-HE Liman et al., 1992 N/A
Plasmid: pcDNA3.1(+) Invitrogen V790-20
Plasmid: FUGW-H1 Addgene #25870
Plasmid: pVSVg Stewart et al., 2003 N/A
Software and Algorithms
ImageJ NIH https://imagej.nih.gov/ij/
GraphPad Prism 8 GraphPad Software https://www.graphpad.com/scientific-software/prism/
LabChart ADInstruments https://www.adinstruments.com/products/labchart