KEY RESOURCES TABLE
| REAGENT or RESOCURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-Tubulin | Sigma | Cat#: T9026; RRID AB_477593 |
| Mouse monoclonal anti-FLAG | Sigma | Cat#: F1804; RRID: AB_262044 |
| Rat monoclonal anti-HA | Roche | Cat#: 11867423001; RRID:AB_390918 |
| Rabbit polyclonal anti-P2X3 | Alomone | Cat#: APR-016; RRID:AB_2313760 |
| Mouse monoclonal anti-NR1 | BD Biosciences | Cat#: 556308; RRID: AB_396353 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Halt protease inhibitor cocktail | ThermoFisher Scientific | Cat#: 78446 |
| GlutaMax | GIBCO | 35050–061 |
| L-Glutamine | GIBCO | 25030–081 |
| Penicillin Streptomycin | GIBCO | 15140–122 |
| poly-D-lysine | Sigma | Cat#: P6407 |
| Protein A Sepharose CL4B | GE Healthcare | Cat#: 17-0780-01 |
| Sulfo-NHS-SS-Biotin | Thermo Scientific | Cat#: 21331 |
| NeutraAvidin Plus Ultralink Resin | Thermo Scientific | Cat#: 53151 |
| FuGENE® 6 Transfection Reagent | Promega | Cat#: E2691 |
| Trypsin-EDTA (0.05%), phenol red | GIBCO | Cat#: 25300054 |
| Advanced DMEM | GIBCO | Cat#: 12491023 |
| Adenosine 5′-triphosphate magnesium salt | Sigma | Cat#: A9187 |
| TNP-ATP triethylammonium salt | Tocris | Cat#: 2464 |
| α, β-Methyleneadenosine 5′-triphosphate lithium salt | Sigma | Cat#: M6517 |
| Ham’s F-12 Nutrient Mix | GIBCO | Cat#: 11765054 |
| NGF 2.5S Native Mouse Protein | GIBCO | Cat#: 13257019 |
| Collagenase P | Roche | Cat#: 11213857001 |
| Fetal Bovine Serum, qualified, USDA-approved regions | GIBCO | Cat#: 10437028 |
| Trypsin-EDTA (0.25%), phenol red | GIBCO | Cat#: 25200056 |
| L-Glutamic acid monosodium salt hydrate | Sigma | Cat#: G5889 |
| (−)-(α)-Kainic Acid (hydrate) | Cayman Chemical Company | Cat#: 78050 |
| Acetylcholine chloride | Sigma | Cat#: A5625 |
| Cyclothiazide | Sigma | Cat#: C9847 |
| HBSS, no calcium, no magnesium, no phenol red | GIBCO | Cat#: 14175-095 |
| MEM Non-Essential Amino Acids Solution (100X) | GIBCO | Cat#: 11140050 |
| Gentamycin | GIBCO | Cat#: 11811-031 |
| Sodium Pyruvate | Sigma | Cat#: P5280-25G |
| Hygromycin B (50 mg/mL) | GIBCO | Cat#: 10687010 |
| Gateway LR Clonase II Enzyme mix | Invitrogen | Cat#: 11791-020 |
| Minimum Essential Medium | GIBCO | Cat#: 11090-081 |
| Collagenase from Clostridium histolyticum | Sigma | Cat#: C9891-1G |
| Trypsin from bovine pancreas | Sigma | Cat#: T1426-1G |
| Deoxyribonuclease I from bovine pancreas | Sigma | Cat#: D4527 |
| Cytosine β-D-arabinofuranoside hydrochloride | Sigma | Cat#: C6645-25MG |
| Insulin-transferrin-sodium selenite media supplement | Sigma | Cat#: I1884-1VL |
| 2-Mercaptoethanol | Sigma | Cat#: M3148-25ML |
| Dithiothreitol | American Bioanalytical | Cat#: AB00490-00010 |
| Basal Eagle’s medium | GIBCO | Cat#: 21010-046 |
| Geneticin | GIBCO | Cat#: 15710-064 |
| Critical Commercial Assays | ||
| mMESSAGE mMACHINE T7 Transcription Kit | Ambion | Cat#: AM1344 |
| Pierce BCA Protein Assay Kit | Pierce | Cat#: 23225 |
| 5Prime Perfectprep® Plasmid 96 VAC Base Kit | 5Prime | Cat#: 2300320 |
| SuperSignal ELISA Femto Maximum Sensitivity Substrate | Thermo Scientific | Cat#: 37074 |
| Experimental Models: Cell Lines | ||
| HEK293 | ATCC | Cat#: CRL-1573 |
| 293FT | Life Technologies | Cat#: R70007 |
| Experimental Models: Organisms/Strains | ||
| Mouse: Wild-type (C57BL/6J) | The Jackson Laboratory | Stock#: 000664 |
| Recombinant DNA | ||
| Plasmid: pGEM-HE | Liman et al., 1992 | N/A |
| Plasmid: pcDNA3.1(+) | Invitrogen | V790-20 |
| Plasmid: FUGW-H1 | Addgene | #25870 |
| Plasmid: pVSVg | Stewart et al., 2003 | N/A |
| Software and Algorithms | ||
| ImageJ | NIH | https://imagej.nih.gov/ij/ |
| GraphPad Prism 8 | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| LabChart | ADInstruments | https://www.adinstruments.com/products/labchart |