. Author manuscript; available in PMC: 2020 Oct 11.

Published in final edited form as: ACS Infect Dis. 2019 Aug 21;5(10):1772–1778. doi: 10.1021/acsinfecdis.9b00228

Table 2.

Kinetic Values of Wild-Type Eis (Eis_wt) and Its Mutants

enzyme	K_m (μM)	k_cat (min⁻¹)	k_cat/K_m (min⁻¹ μM⁻¹)
Eis_wt^a	551 ± 134	14.3 ± 1.9	0.026 ± 0.007
Eis_D26A	1280 ± 360	14.1 ± 2.5	0.011 ± 0.004
Eis_W36A	556 ± 148	8.3 ± 1.0	0.015 ± 0.004
Eis_W36R	352 ± 77	4.4 ± 0.4	0.013 ± 0.003
Eis_R37A^a,c	971 ± 166	9.5 ± 0.3	0.010 ± 0.002
Eis_R37G^b,c	696 ± 44	12.9 ± 0.1	0.019 ± 0.001
Eis_L63A^b,c	484 ± 93	19.4 ± 1.5	0.040 ± 0.008
Eis_M65A^c	247 ± 19	29.7 ± 0.9	0.122 ± 0.015
Eis_S83G^b	432 ± 48	21.9 ± 0.8	0.051 ± 0.006
Eis_F84A	569 ± 94	8.5 ± 0.7	0.015 ± 0.003

Average for three sets of assays listed in Table S2.

Average for two sets of assays listed in Table S2.

A residue that was not observed to interact with TOB (Table 1).