Table 3.

IC₅₀ Values for the Inhibitors of Interest Measured for Eis_wt and Its Mutants

enzyme	inhibitor	IC50 (μM)	Ki (μM)
Eis_wt	1	0.088 ± 0.012	0.048 ± 0.003
	2	0.197 ± 0.025	0.095 ± 0.005
	3	0.093 ± 0.02	0.096 ± 0.010
Eis_D26A	1	>200	–a
	2	>200	–
	3	>200	–
Eis_W36A	1	>200	–
	2	>200	–
	3	>200	–
Eis_W36R	1	0.046 ± 0.016	0.037 ± 0.004
	2	>200	–
	3	>200	–
Eis_R37A	1	0.388 ± 0.071	0.175 ± 0.037
	2	0.301 ± 0.047	0.197 ± 0.017
	3	0.252 ± 0.024	0.185 ± 0.015
Eis_R37G	1	>200	–
	2	0.975 ± 0.080	0.351 ± 0.047
	3	1.72 ± 0.21	0.349 ± 0.039
Eis_L63A	1	>200	–
	2	0.227 ± 0.018	0.147 ± 0.014
	3	0.148 ± 0.016	0.063 ± 0.006
Eis_M65A	1	0.643 ± 0.099	0.122 ± 0.015
	2	>200	–
	3	>200	–
Eis_S83G	1	0.880 ± 0.080	1.03 ± 0.09
	2	>200	–
	3	0.118 ± 0.017	0.060 ± 0.006
Eis_F84A	1	>200	–
	2	>200	–
	3	>200	–

^aK_i measurements were not carried out for IC₅₀ > 200 μM due to very weak inhibitor binding.