Propionic acid is converted to propionyl-CoA (P-CoA) in normal control hepatocytes. A. Propionic acid (PA) at 0, 0.3. 1, 3, 10 mM is converted to P-CoA in a dose-responsive fashion (1 donor, N=4 biological replicates/dose, N=1 technical replicates/biological replicate). B. Addition of 10 mM PA to normal hepatocytes increases the TCA cycle intermediates downstream of its entrance at succinyl-CoA (succinate, fumarate, malate, oxaloacetate, pyruvate and citrate). It does not increase the level of isocitrate, alpha-ketoglutarate or 2-hydroxyglutarate (1 donor, N=7 biological replicates, N=1 technical replicates/biological replicate). Error bars represent standard error of the mean.