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. 2020 Jul 6;15(7):e0235852. doi: 10.1371/journal.pone.0235852

Fig 3. ACM effects on CD133 surface expression and neurosphere formation.

Fig 3

(A) Percentage of CD133 positive cells cultured in ACM or DMEM (control), measured by flow cytometry at 24, 48, and 72 h. (B) Percentage of CD15 positive cells cultured in ACM or DMEM (control), measured by flow cytometry at 24, 48, and 72 h. (C) Representative images of Daoy cells cultured in neurosphere conditions using the respective media for 48 h. Scale bar is 100 μm. (D) Total number of neurospheres counted in all images between 75–100 μm or >100 μm at 48 h, cultured in the respective media. (E) Western blot analysis of Nestin, (F) Oct-4A and (G) Sox2 expression in respective neurosphere culturing conditions at 48 h. β-actin served as a loading control (left). Quantification of relative fold change of Nestin, Oct-4A and Sox2 expression, measured by the densitometry of western blot bands and normalized to β-actin (DMEM condition set to 1) (right). Quantified values expressed as mean +/- SD. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.