Table 1. In vitro incubation with high glucose concentration significantly enhances erythrocyte glycation.

The impact of erythrocyte incubation in the presence of enhanced glucose concentration on the percentage of glycated hemoglobin (% HbA1c), the 5-hydroxymethylfurfural and the early glycation product (EGP) levels were determined as described in material and method section. GX corresponds to erythrocytes incubated with X mM glucose and G0 corresponds to erythrocytes incubated in the absence of glucose. Data are expressed as mean ± SEM (n = 6 to 8 independent replicates) and statistical analyses were performed using One-way ANOVA followed by Dunnett’s test. *p<0.05, **p<0.01 in comparison with G0.

	G0			G5			G25			G137
HbA1c (%)	5.22	±	0.4	5.18	±	0.4	4.9	±	0.47	7.06	±	1.65*
5-HMF (% G0)	100	±	14.4	97.2	±	10.1	102	±	14.8	155	±	31.5**
EGP (% G0)	100	±	19.3	129.8	±	14.4	135.3	±	20.4	457	±	63.2**