Table 1.
Summary of cryo-EM data collection, 3D reconstruction, and model refinement
| SARS-Covid19 Spike (D614G) | |
|---|---|
| Imaging parameters and 3D reconstruction | |
| Cryo-EM grids | UltrAuFoil R1.2/1.3 |
| Calibrated magnification | 155000 |
| Acceleration voltage [kV] | 300 |
| Pixel size [Å] | 0.52 |
| Total dose [e−/Å2] | 37 |
| Exposure time [s] | 1.81 |
| Defocus range [μm] | −0.6 ~ −1.8 |
| Particles in final 3D reconstruction | 266356 |
| Resolution (‘Gold-standard’ at FSC 0.143) [Å] | 3.7 |
| Model refinement | |
| Resolution in phenix.real_space_refine [Å] | 4.0 |
| No. atoms: protein | 18294 |
| Ligands / ions | N/A |
| r.m.s. deviations: bond lengths [Å] | 0.004 |
| r.m.s. deviations: bond angles [°] | 0.808 |
| MolProbity score | 4.49 |
| Rotamer outliers [%] | 0.00 |
| Ramachandran angles: favored [%] | 93.88 |
| Ramachandran angles: allowed [%] | 6.12 |
| Ramachandran angles: outliers [%] | 0.00 |
| PDB | 6xs6 |
| EMDB | EMD-22301 |