Table 2.
List of Reagents and Resources used in this manuscript
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-SARS-CoV-2 M protein | ThermoFisher | PA1–41160 |
| Rabbit polyclonal anti-SARS-CoV-2 E protein | Antibodies-online.com | ABIN1031551 |
| Rabbit polyclonal anti-SARS-CoV-2 N protein | Rockland Immunochemicals | 200–401-A50 |
| Rabbit monoclonal anti-SARS-CoV-2 S protein (S1-specific) | GeneTex | GTX635656 |
| Rabbit monoclonal anti-SARS-CoV-2 S protein (S1-specific) | GeneTex | GTX635672 |
| Mouse/human monoclonal anti-SARS-CoV-2 S protein (S2-specific) | Sino Biological | 40590-D001 |
| Rabbit polyclonal anti-Raptor | EMD Millipore | 09–217 |
| Goat anti-rabbit antibody 800CW | Licor | 925–32211 |
| Goat anti-mouse 680RD | Licor | 925–68070 |
| Goat anti-rabbit, HRP-linked antibody | Cell Signaling Technology | 7074 |
| Goat anti-mouse, HRP-linked antibody | Cell Signaling Technology | 7076 |
| Human monoclonal antibody targeting the receptor binding domain of the SARS-CoV-2 S protein |
A. Baum et al., Sciencel 0.1126/science.abd0831 (2020)
J. Hansen et al., Sciencel 0.1126/science.abd0827 (2020) |
REGN10987 |
| Human monoclonal antibody targeting the receptor binding domain of the SARS-CoV-2 S protein |
A. Baum et al., Sciencel 0.1126/science.abd0831 (2020)
J. Hansen et al., Sciencel 0.1126/science.abd0827 (2020) |
REGN10989 |
| Human monoclonal antibody targeting the receptor binding domain of the SARS-CoV-2 S protein |
A. Baum et al., Sciencel 0.1126/science.abd0831 (2020)
J. Hansen et al., Sciencel 0.1126/science.abd0827 (2020) |
REGN10933 |
| Human monoclonal antibody targeting the receptor binding domain of the SARS-CoV-2 S protein |
A. Baum et al., Sciencel 0.1126/science.abd0831 (2020)
J. Hansen et al., Sciencel 0.1126/science.abd0827 (2020) |
REGN10934 |
| Mouse monoclonal, anti-human Fc antibody |
A. Baum et al., Sciencel 0.1126/science.abd0831 (2020)
J. Hansen et al., Sciencel 0.1126/science.abd0827 (2020) |
REGN2567 |
| Viruses | ||
| VSVΔG:mNeon/VS V-G virus |
A. Baum et al., Sciencel 0.1126/science.abd0831 (2020)
J. Hansen et al., Sciencel 0.1126/science.abd0827 (2020) |
N/A |
| Chemicals, Peptides, Commerical Assays, and Recombinant Proteins | ||
| hsACE2.hFc |
A. Baum et al., Sciencel 0.1126/science.abd0831 (2020)
J. Hansen et al., Sciencel 0.1126/science.abd0827 (2020) |
N/A |
| blasticidin | InvivoGen | ant-bl-1 |
| puromycin | InvivoGen | ant-pr-1 |
| gentamicin | Life Technologies | 15750078 |
| Ni-NTA resin | EMD Millipore | 30230 |
| Superose 6 gel-filtration column | GE Healthcare | 29091596 |
| Mycoplasma detection kit | Lonza | LT07–318 |
| PEI | Polysciences, Inc. | 23966–1 |
| TransIT LT1 transfection reagent | Mirus Bio | MIR 2304 |
| Lipofectamine LTX | Life Technologies | 15338100 |
| Steady-Glo Luciferase Assay System | Promega | E2550 |
| Cytofix | BD Biosciences | 554655 |
| SMM-293 Til serum-free media | SinoBiological | M293TI |
| Deposited Data | ||
| PDB | 6xs6 | N/A |
| EMDB | EMD-22301 | |
| Cell Lines | ||
| Human: HEK-293 cells | ATCC | CRL-1573 |
| Human: HEK-293T cells | ATCC | CRL-3216 |
| Human: HEK-293T/17 cells | ATCC | CRL-11268 |
| Human: Freestyle 293-F cells | ThermoFisher | R79007 |
| Human: Calu-3 cells | ATCC | HTB-55 |
| Human: Caco-2 cells | ATCC | HTB-37 |
| Human: SUPT1 [VB] cells | ATCC | CRL-1942 |
| Cercophithecus aethiops: Vero cells | ATCC | CCL-81 |
| Recombinant DNA | ||
| pcDNA3.1 SARS-CoV-2 S D614 | This publication | Addgene #158074 |
| pcDNA3.1 SARS-CoV-2 S D614G | This publication | Addgene #158075 |
| pcDNA3.1 SARS-CoV-2 S D614-trunc | This publication | Addgene #158076 |
| pcDNA3.1 SARS-CoV-2 S D614G-trunc | This publication | Addgene #158077 |
| pcDNA3.1 SARS-CoV-2 M | This publication | Addgene #158078 |
| pcDNA3.1 SARS-CoV-2 N | This publication | Addgene #158079 |
| pcDNA3.1 SARS-CoV-2 E | This publication | Addgene #158080 |
| pscALPSpuro | Addgene | Cat# 128504 |
| pscALPSpu ro-HsA CE2 (human) | This publication NP_001358344.1 | Addgene #158081 |
| pscALPSpu ro-FcA CE2 (Cat) | This publication XP_023104564.1 | Addgene #158082 |
| pscALPSpu ro-ClAC E2 (Dog) | This publication NP_001158732.1 | Addgene #158083 |
| pscALPSpu ro-MjA CE2 (Pangolin) | This publication XP_017505752 | Addgene #158084 |
| pscALPSpu ro-SsA CE2 (Pig) | This publication NP_001116542.1 | Addgene #158085 |
| pscALPSpu ro-RnA CE2 (Rat) | This publication NP_001116542.1 | Addgene #158086 |
| pscALPSpu ro-MmA CE2 (Mouse) | This publication NP_001116542.1 | Addgene #158087 |
| pscALPSblasti-GFP | Addgene | Cat# 115804 |
| pscALPSblasti-TM PRSS2 Blasti | This publication | Addgene #158088 |
| HIV-1 pNL4–3 Δenv Δvpr luciferase reporter vector (pNL4–3.Luc.R-E-) | NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH: from Dr. Nathaniel Landau | Cat #3418 |
| pUC57mini NL4–3 Δenv eGFP | This publication | Pending NIH AIDS Reagent Program |
| psPAX2 | Addgene | Cat #12260 |
| pMD2.G | Addgene | Cat# 12259 |
| pCAGGS-ACE2-his | This publication | Addgene #158089 |
| Software and Algorithms | ||
| FlowJo | FlowJo LLC; BD | N/A |
| Prism 8.4.3 | GraphPad | N/A |
| T200 Evaluation software v 3.1 | Biacore | N/A |
| Scrubber 2.0 | BioLogic Software | N/A |
| Coot | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ | Version 0.9 |
| PHENIX | https://www.phenix-online.org/ | Version 1.18.2 |
| MolProbity | http://molprobity.biochem.duke.edu/ | Version 4.5.1 |
| MAFFT v7.464 | Katoh and Standley, 2013 | https://mafft.cbrc.jp/alignment/software/ |
| Biopython v1.76 | Cock et al., 2009 | https://biopython.org/ |
| Matplotlib v3.2.2 | Hunter, 2007 | https://matplotlib.org/ |
| SnpEff v4.5covid19 | Cingolani, et al. 2012 | http://snpeff.sourceforge.net/ |
| bcftools v1.9 | Li et al., 2009 | http://samtools.github.io/bcftools/bcftools.html |
| snp-sites v2.5.1 | Page, et al. 2016 | https://sanger-pathogens.github.io/snp-sites/ |
| EPU 2 software for single particle analysis | Thermo Fisher Scientific | N/A |
| Hardware, Machines, and Other | ||
| Quantifoil Au grid | Quantifoil, Großlöbichau Germany | NA |
| GloQube Plus Glow Discharge System | Quorumtech | N/A |
| Vitrobot Mark IV | Thermo Fisher Scientific | N/A |
| 300 kV Krios transmission electron microscope | Thermo Fisher Scientific | N/A |