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[Preprint]. 2020 Jul 1:2020.07.01.182659. [Version 1] doi: 10.1101/2020.07.01.182659

Figure 2.

Figure 2

SARS-CoV-2 RBD but not SARS-CoV RBD enabled ACE2 to cleave bradykinin analog. A) and B) hydrolysis of Mca-YVADAPK-Dnp(20uM) in the presence of SARS-CoV-2 RBD and SARS-CoV RBD proteins at indicated concentrations. C) and D) hydrolysis of Mca-RPPGFSAFK-Dnp (20uM) in the presence of SARS-CoV-2 RBD and SARS-CoV RBD proteins at indicated concentrations. E) Fold change of RFU during Mca-YVADAPK-Dnp cleavage in the presence of SARS-CoV-2 RBD or SARS-CoV RBD at the time point of 1.5h due to instrument overflow. F) Fold change of RFU during Mca-RPPGFSAFK-Dnp cleavage in the presence of SARS-CoV-2 RBD or SARS-CoV RBD at time point of 8h. All RFU readings at different concentrations of RBD proteins were normalized to that of ACE2 cleavage without RBD proteins correspondingly.