Skip to main content
[Preprint]. 2020 Jul 1:2020.07.01.182659. [Version 1] doi: 10.1101/2020.07.01.182659

Figure 3.

Figure 3

Measurement of kinetic constants for hydrolysis of Mca-RPPGFSAFK-Dnp and Mca-YVADAPK-Dnp in the absence or presence of SARS-CoV-2 spike protein (14ug/ml). A) and B) background subtracted RFU kinetic readings during ACE2 hydrolysis of Mca-RPPGFSAFK-Dnp at different concentrations in the absence (A) and presence (B) of SARS-CoV-2 spike protein. C) and D) background subtracted RFU kinetic readings during ACE2 hydrolysis of Mca-YVADAPK-Dnp at different concentrations in the absence (C) and presence (D) of SARS-CoV-2 spike protein. E) and F) Michaelis plots for ACE2 hydrolysis of Mca-RPPGFSAFK-Dnp and Mca-YVADAPK-Dnp in the absence (blue) or presence (green)of SARS-CoV-2 spike protein. The initial velocity conditions were limited to 30min for Mca-YVADAPK-Dnp and 60min for Mca-RPPGFSAFK-Dnp due to different cleavage rate. All determinations were repeated with duplicates.