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. 2020 Jun 9;219(7):e201911047. doi: 10.1083/jcb.201911047

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© 2020 Shi et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 5. — The ULK complex has unequal subunit stoichiometry. (A) Negative stain 2D class averages of FIP200NTD:MBP-ATG13MR complex. Densities corresponding to MBP tags are labeled with yellow arrows. Scale bar is 20 nm. (B) Single particles of FIP200NTD:MBP-ATG13:MBP-ATG101:ULK1 EAT complex. Density for dual MBP-tagged HORMA domains is highlighted with yellow arrows. Scale bar is 20 nm. (C) 2D class averages of FIP200NTD:ATG13-MBP:ATG101:ULK1 EAT–MBP complex. Densities corresponding to MBP tags are labeled with yellow arrows. Scale bar is 20 nm. (D and E) MALS traces for FIP200NTD:MBP-ATG13MR (D) and FIP200NTD:ATG13(363-C):ULK1 EAT–MBP (E). MW, molecular weight.