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. 2020 Jun 9;219(7):e201911047. doi: 10.1083/jcb.201911047

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© 2020 Shi et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 6. — Cryo-EM reconstruction of the FIP200NTD:ATG13MR complex and model for ULK complex assembly. (A) Cryo-EM 2D class averages of FIP200NTD:ATG13MR. Scale bar is 20 nm. (B) 3D reconstructions of dimeric FIP200NTD overlaid with measurements from tip-to-tip ranging from 164 Å to 218 Å across. The different colors correspond to different density maps from different 3D classes. (C) Views of masked final 3D reconstruction of FIP200NTD monomer. (D) Fit of TBK1 structure into the final map (left) and colored by ULD (green) and SLD (right; blue). (E) Model for asymmetric FIP200 ULK complex assembly and proposed formation of higher oligomers during autophagy initiation.