Figure S3.

Mitochondrial morphology rescue assay. (A) The mitochondrial morphology in WT or OPA1-deleted MEF cells was visualized by indirect immunofluorescence using anti-Tom20 antibodies. The nuclei are stained with Hoechst. Scale bars, 10 µm. (B) EM images of WT and OPA1-deleted MEF cells. Representative mitochondria are shown. Scale bars, 0.5 µm. (C) Myc-tagged WT OPA1 was transfected into OPA1-deleted MEF cells. Localization was determined using anti-Myc antibodies (green) and compared with mitochondrial marker Tom20 by confocal microscopy. Scale bars, 10 µm. (D) Expression of OPA1 used in Fig. 5 F is shown with actin as a loading control. (E) EM images of cells used in Fig. 5 F. The cristae morphology is scored as indicated. Scale bar, 0.2 µm.