Skip to main content
. 2020 May 21;219(7):e201912098. doi: 10.1083/jcb.201912098

Figure S4.

Figure S4.

PI3KC3-C1 is active on unsaturated flat membranes. (A) Representative confocal images of GUVs showing the binding of the PI3KC3-C1 complex and FYVE domain on different membranes. mCherry-FYVE domain (1 µM) was incubated with GUVs with brain lipids (64.8% brain PC:20% brain PE:5% brain PS:10% liver PI:0.2% Atto647 DOPE), PO lipids (64.8% POPC:20% POPE:5% POPS:10% POPI:0.2% Atto647 DOPE), or DO lipids (64.8% DOPC:20% DOPE:5% DOPS:10% POPI:0.2% Atto647 DOPE) in the absence or presence of GFP-tagged PI3KC3-C1 (200 nM) for 30 min. (B) Quantification of the relative intensities of PI3KC3-C1 on different GUV membranes (means ± SD; n = 50). P values were calculated using Student’s t test: ****, P < 0.0001. AU, arbitrary units. (C) Quantification of the relative intensities of FYVE domain on different GUV membranes (means ± SD; n = 50). P values were calculated using Student’s t test: ****, P < 0.0001. AU, arbitrary units.