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WIPI3 mediates PI3KC3-C1 trigged LC3 lipidation on GUV membranes. (A) Representative confocal images of GUVs showing E3 membrane recruitment and LC3B lipidation. mCherry-tagged LC3B was incubated with PI3KC3-C1 (100 nM), WIPI3 (400 nM), E3-GFP, ATG7, and ATG3 in the presence or absence of ATP. (B) Quantitation of the kinetics of E3 recruitment and LC3B lipidation on the membrane from individual GUV tracing in (A) (means ± SD; n = 20; 15). AU, arbitrary units.
