Figure 1.

Whi3 is differentially phosphorylated during nocodazole arrest and heat stress. (A) Representative images of Whi3-tomato protein localized in hyphae under different growth conditions. Scale bar, 5 µm. (B) Normalized Whi3 abundance as measured by fluorescence intensity of Whi3-tomato protein within the hypha under different growth conditions. n > 386 for all conditions. *, P < 0.05 by one-way ANOVA with multiple comparisons. Bars denote 95% confidence interval. ***, P < 0.0005. (C) Percent of hyphal tips containing a Whi3 punctum. n > 62 for all conditions. *, P < 0.05 by N-1 χ² test with Benjamini–Hochberg correction. (D) Average number of Whi3 puncta per 5.410-µm hyphal segment. n > 66 for all conditions. *, P < 0.05 by one-way ANOVA with multiple comparisons. Bars denote 95% confidence interval. (E) Schematic of mass spectrometry workflow. (F) Diagram of identified or predicted phosphorylation sites. RRM, RNA-recognition motif; AU, arbitrary units; n.s., not significant