Figure 2.

Single point mutants at sites of Whi3 phosphorylation can increase nuclear synchrony. (A) Nuclei in wild-type A. gossypii cells go through the cell cycle asynchronously but can become more synchronous in certain genetic backgrounds. Nuclei cartooned in cell cycle progression shown with spindle pole bodies. (B) Representative images of SPB labeling for synchrony scoring in control (asynchronous), 111D (increased synchrony mutant), and 637A (asynchronous mutant). SPBs are in red and nuclei are in cyan. Hyphal outlines are dotted lines and representative nuclei of each nuclear cycle stage are indicated with arrows in control cell. Images are maximum intensity projections. Scale bar, 5 µm. (C) Synchrony indices for Whi3 mutants as compared with control cells. n > 180 for all conditions. Bars denote SE. *, P < 0.05. (D) Percent of nuclei in each phase of the nuclear cycle in control and Whi3 mutant strains. n > 180 for all conditions. G1 nuclei are orange bars, S/G2 nuclei are blue bars, and M nuclei are pink bars. Synchrony phenotype for each strain is represented by cartoon image below strain name. n.s., not significant; SPB, spindle pole body.