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. 2020 May 12;219(7):e201910021. doi: 10.1083/jcb.201910021

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© 2020 Gerbich et al.

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Figure 3. — Single point mutants at potential sites of Whi3 phosphorylation can disrupt cellular polarity and lateral branching. (A) Representative images to show gross morphology of control and Whi3 mutant strains grown on agar pads. Cartoon representation of polarity phenotype for each strain is shown below each strain. Scale bar, 50 µm. (B) Montage of cells growing on plate for control (normal polarity), 111D (normal polarity mutant), and 637A (disrupted polarity mutant). Interval between frames is 108 min. Scale bar, 50 µm. (C) Representative images of actin at hyphal tips in control and selected Whi3 mutant strains. Images are maximum intensity projections. Scale bar, 2 µm. (D) Length of polarized actin zone along hyphal long axis in control and Whi3 mutant strains. n > 31 for all conditions. *, P < 0.05 by one-way ANOVA with multiple comparisons. **, P < 0.005; ***, P < 0.0005. Bars, 95% confidence interval.