Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 12;219(7):e201910021. doi: 10.1083/jcb.201910021

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Gerbich et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 4. — Single point mutants at potential sites of Whi3 phosphorylation can strongly influence Whi3 localization and assembly. (A) Representative images of Whi3 protein in control or Whi3 mutant strains localized in the hypha. Images are maximum intensity projections. Scale bar, 5 µm. Tom, Tomato. (B) Representative images of hyphal tips containing or not containing Whi3-tomato puncta. Images are maximum intensity projections. Scale bar, 2 µm. (C) Normalized Whi3 abundance in control and Whi3 mutant cells under different growth conditions. n > 62 for all conditions. *, P < 0.05 by one-way ANOVA with multiple comparisons. Bars, 95% confidence interval. (D) Percent of hyphal tips in control and Whi3 mutant strains containing a Whi3 punctum. n > 167 for all conditions. *, P < 0.05 by N-1 χ² test with Benjamini–Hochberg correction. (E) Average number of Whi3 puncta per 10-µm hyphal segment in control and Whi3 mutant strains. *, P < 0.05 by one-way ANOVA with multiple comparisons. **, P < 0.005; ***, P < 0.0005. Bars, 95% confidence interval.