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. 2020 May 20;48(12):6824–6838. doi: 10.1093/nar/gkaa340

Figure 2.

Figure 2.

RBM10 regulates SAT1 alternative splicing and modulates DENV infection (A, B) Quantified inclusion/exclusion ratio in A549 cells either transfected with siRNA against RBM10 (A) or an expression vector for T7-RBM10 (B). (C) RNA from A549 cells transfected with the indicated siRNAs or DNA plasmids for 24 h and infected with DENV for additional 48 h was quantified by RT-qPCR for SAT1 inclusion/exclusion ratio. (D) RBM10 knock-down and over-expression in A549 infected cells were monitored by western blot (M: Mock; DV: DENV). (E) Viral infection was monitored by RT-qPCR of viral RNA and by plaque formation assay (PFU: Plaque Forming Units). Average values from triplicates are shown with standard deviation and P-values, determined using a paired two-tailed t-test. Significant p-values are indicated by the asterisks above the graphs (***P< 0.001; **P< 0.01; *P< 0.05). (F) Model of SAT1 pre-mRNA splicing regulation upon RBM10 protein level alterations or DENV infection.