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. 2020 May 20;48(12):6824–6838. doi: 10.1093/nar/gkaa340

Figure 4.

Figure 4.

RBM10 is involved in the anti-viral innate response. (A–C) A549 cells were transfected with the indicated siRNAs (40 nM) or expression vectors (200 ng) for 24 h and infected with DENV for additional 48 h. RIG-I, IFN α, IL-8 and ISG15 mRNA levels were analyzed by RT-qPCR (A, B) and RBM10 and tubulin protein levels by western blot (M: Mock; DV: DENV) (C). (D) A549 cells were transfected with the indicated siRNAs (40 nM) for 72 h during which, the last 9 h included Poly I:C (10 μg /ml) intracellular administration. Upon harvesting, RNA was prepared and analyzed by RT-qPCR for ISG15 and IFIT1 mRNAs. Average values from triplicates are shown with standard deviation and P-values, determined using a paired two-tailed t-test. Significant P-values are indicated by the asterisks above the graphs (***P< 0.001; **P< 0.01; *P< 0.05).