Figure 4.

Influence of additional AUG codons in the ramp mRNA region on the yield of translation. Schematic representation (left side of the panel) and translation efficiencies (right side of the panel) of the constructs. All constructs designations are indicated next to the schematic representations. AUG codons are shown. Red boxes correspond to the out of frame AUG codons, while that in the CER frame are shown in green boxes. Translation efficiencies of the CER reporter were normalized to the reference RFP construct and indicated as a diagram. Exact values are shown next to the corresponding bars. The sequences are listed in Supplementary Table S2.