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. Author manuscript; available in PMC: 2020 Nov 19.
Published in final edited form as: Anal Chem. 2019 Nov 1;91(22):14569–14576. doi: 10.1021/acs.analchem.9b03625

Figure 2:

Figure 2:

MRM EICs for nucleobase standards using a) C18 column with reversed-phase chromatography, b) PGC column, or c) mixed-mode cation-exchange with C18 column using a pH and organic gradient elution.