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Purines and pyrimidines release from RNA – hydrolysis timecourse using gas-phase formic acid at 140°C. Purines (adenine and guanine) are rapidly released from RNA (or DNA) and can be quantified within 2 hours of hydrolysis, while pyrimidines (cytosine and uracil) do not reach complete hydrolysis until more than 24 hours after hydrolysis begins.
