Figure 2.

Infection of SARS-CoV-2 in moDCs and MDMs was not productive. MoDCs (A) and MDMs (B) were infected with SARS-CoV-2 or SARS-CoV at an MOI of 1. The live infectious virus particles in the supernatants and the viral genome copy in the cell lysates were determined by plaque assays and qRT-PCR, respectively. C, The cell viability of moDCs and MDMs upon SARS-CoV-2 or SARS-CoV infection at an MOI of 1 was quantified at the indicated hours post infection using CellTiterGlo assays. The mean cell viability of SARS-CoV-2– or SARS-CoV–infected cells was compared with that of mock-infected cells at each time point. The results represent mean and standard deviations from 3 individual donors in 3 independent experiments. Statistical significance between the groups was determined with 1-way ANOVA and was consider significant when P < .05. Abbreviations: MOI, multiplicity of infection; PFU, plaque-forming unit; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; moDCs, monocyte-derived dendritic cells; MDMs, monocyte-derived macrophages; qRT-PCR, quantitative reverse transcription polymerase chain reaction; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2.