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. 2020 Jun 21;222(5):734–745. doi: 10.1093/infdis/jiaa356

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© The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.

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Figure 3. — SARS-CoV-2 modulated the differential expression of entry-related host factors in moDCs and MDMs. A, The expression of endogenous ACE2, TMPRSS2, FURIN, and DC-SIGN was evaluated in moDCs and MDMs. Calu3 cells were included as a positive control. B, MoDCs and MDMs were infected with SARS-CoV-2 or SARS-CoV at an MOI of 1. The cell lysates were harvested to detect expression of entry-related host factors using qRT-PCR at the indicated hours post infection. The expression of entry-related host factors in SARS-CoV-2–infected or SARS-CoV–infected cells was compared with that of mock-infected cells at each time point. The results represent mean and standard deviations from 3 to 6 individual donors in 3 independent experiments. Statistical significance between groups was determined with 1-way ANOVA and was consider significant when P < .05. * P < .05, ** P < .01, *** P < .001, **** P < .0001. Abbreviations: MOI, multiplicity of infection; ACE2, angiotensin-converting enzyme 2; TMPRSS2; transmembrane protease serine 2; DC-SIGN, dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin; moDCs, monocyte-derived dendritic cells; MDMs, monocyte-derived macrophages; qRT-PCR, quantitative reverse transcription polymerase chain reaction; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2.