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. 2020 Jun 21;222(5):734–745. doi: 10.1093/infdis/jiaa356

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© The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.

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Figure 6. — SARS-CoV-2 antagonized STAT1 phosphorylation in moDCs. MoDCs and MDMs were mock-infected or infected with SARS-CoV-2 or SARS-CoV at an MOI of 10. At 24 hours post infection, the cells were untreated or treated with 1000 U/mL of recombinant human IFN-α for 40 minutes. The cell lysates were collected for the detection of STAT1, pSTAT1, and β-actin by Western blots. A, Representative blots are shown from 3 donors in 3 independent experiments. B, Quantitation was calculated as the ratio of pSTAT1 over STAT1 protein. Statistical analysis was performed with 1-way ANOVA and the differences were considered significant when P < .05. *P < .05, ****P < .0001. Abbreviations: MOI, multiplicity of infection; IFN, interferon; moDCs, monocyte-derived dendritic cells; MDMs, monocyte-derived macrophages; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2.