Table 2.
Re-evaluation of mutagenic capability of Mu insertions by RNAseq and sequencing RT-PCR products via Miseq
| Affected_gene | Insertion_site | B73_insertion | Mo17_no_insertion | B73_no_insertion | Mo17_no_insertion | Knockdown_index | Consequence |
|---|---|---|---|---|---|---|---|
| Zm00001d020901 | five_prime_UTR | 0 | 247 | 461 | 519 | ∞ | knockout |
| Zm00001d027950 | intron | 2 | 517 | 221 | 285 | 200.5 | knockout |
| Zm00001d039733 | five_prime_UTR | 39 | 960 | 553 | 480 | 28.4 | knockdown |
| Zm00001d044446 | five_prime_UTR | 31 | 703 | 148 | 144 | 23.3 | knockdown |
| Zm00001d028712 | five_prime_UTR | 270 | 510 | 192 | 30 | 12.1 | knockdown |
| Zm00001d006460 | five_prime_UTR | 86 | 102 | 50 | 5 | 11.9 | knockdown |
| Zm00001d017424 | five_prime_UTR | 6 | 34 | 819 | 1065 | 4.4 | weak_knockdown |
| Zm00001d005587 | five_prime_UTR | 10 | 60 | 81 | 122 | 4 | weak_knockdown |
| Zm00001d023962 | five_prime_UTR | 78 | 488 | 181 | 463 | 2.4 | weak_knockdown |
| Zm00001d008642 | five_prime_UTR | 119 | 1235 | 361 | 1782 | 2.1 | weak_knockdown |
| Zm00001d006126 | intron | 83 | 68 | 144 | 50 | 2.4 | weak_knockdown |
| Zm00001d006768 | promoter -65 | 63 | 93 | 79 | 62 | 1.9 | unchanged |
| Zm00001d033167 | promoter-117 | 154 | 69 | 140 | 112 | 0.6 | unchanged |
| Zm00001d039683 | promoter-32 | 206 | 95 | 166 | 86 | 0.9 | unchanged |
| Zm00001d012812 | promoter-78 | 752 | 405 | 239 | 138 | 0.9 | unchanged |
| Zm00001d039156 | five_prime_UTR | 455 | 838 | 542 | 676 | 1.5 | unchanged |
| Zm00001d047761 | five_prime_UTR | 161 | 393 | 50 | 83 | 1.5 | unchanged |
| Zm00001d018461 | five_prime_UTR | 402 | 818 | 223 | 383 | 1.2 | unchanged |
| Zm00001d022153 | five_prime_UTR | 320 | 208 | 122 | 79 | 1 | unchanged |
| Zm00001d034667 | five_prime_UTR | 924 | 854 | 288 | 302 | 0.9 | unchanged |
| Zm00001d034191 | five_prime_UTR | 138 | 96 | 36 | 30 | 0.8 | unchanged |
| Zm00001d028784 | five_prime_UTR | 27 | 20 | 355 | 349 | 0.8 | unchanged |
| Zm00001d050081 | five_prime_UTR | 53 | 53 | 31 | 42 | 0.7 | unchanged |
| Zm00001d039253 | five_prime_UTR | 30 | 184 | 16 | 133 | 0.7 | unchanged |
| Zm00001d001788 | five_prime_UTR | 233 | 178 | 497 | 563 | 0.7 | unchanged |
| Zm00001d029856 | intron | 189 | 314 | 104 | 96 | 1.8 | unchanged |
| Zm00001d050163 | intron | 8 | 11 | 127 | 110 | 1.6 | unchanged |
| Zm00001d053452 | intron | 963 | 1324 | 236 | 310 | 1 | unchanged |
| Zm00001d049619 | intron | 70 | 260 | 251 | 1275 | 0.7 | unchanged |
| Zm00001d006610 | three_prime_UTR | 336 | 292 | 288 | 232 | 1.1 | unchanged |
| Zm00001d029059 | five_prime_UTR | 244 | 131 | 46 | 59 | 0.4 | unchanged/weak_activation |
| Zm00001d022122 | intron | 43 | 29 | 2 | 9 | 0.1 | unchanged/weak_activation |