Figure 1.

Genome-wide profiling of HrrA binding in response to addition of external heme. (A) ChAP-Seq analysis of the Corynebacterium glutamicum strain ATCC 13032::hrrA-C-twinstrep grown in iron-depleted glucose minimal medium before and after addition of 4 μM hemin. The experimental approach is briefly depicted: cells were harvested at the indicated time points, twin-Strep tagged HrrA was purified and co-purified DNA was sequenced to identify HrrA genomic targets. This approach resulted in the identification of more than 200 genomic regions bound by HrrA upon addition of hemin after 30 min. Exemplarily shown is the HrrA binding to regions upstream of operons involved in (B) the respiratory chain (ctaE), (C) heme degradation (hmuO) and (D) heme biosynthesis (hemE).