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. 2020 May 26;48(12):6547–6562. doi: 10.1093/nar/gkaa415

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Differential gene expression analysis of wild-type Corynebacterium glutamicum and a ΔhrrA mutant. (A) Differential gene expression analysis (RNA-Seq) revealed 120 upregulated and 154 downregulated genes in the hrrA deletion strain compared to the wild-type (in transcripts per million, TPM) after 30 min of cultivation in iron-depleted glucose minimal medium containing 4 μM heme. (B) Expression levels of hrrA (TPM) 0, 0.5 and 4 h after the addition of hemin. A scheme depicts HrrA autoregulation and iron-dependent DtxR repression (24). (C) Impact of hrrA deletion on the transcript levels of six selected target genes at three different time points (0, 0.5, 4 h; orange: HrrA acts as a repressor, turquoise: HrrA acts as an activator). (D) Measurement of cytochrome aa₃ oxidase activity using the TMPD oxidase assay in C. glutamicum wild-type and ΔhrrA grown with or without 4 μM heme.