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. 2020 May 26;48(12):6547–6562. doi: 10.1093/nar/gkaa415

Table 2.

Apparent Kdvalues of HrrA to the promoters of hmuO, ctaE, sigC and cydA

Promoter Function Apparent Kd value (nM) 95% confidence interval (nM) R² Peak intensity after hemin addition (ChAP-Seq)
PhmuO Heme oxygenase 196 182–212 0.95 10
PctaE Cytochrome aa3 oxidase 125 117–132 0.97 53
PsigC ECF sigma factor σC 271 247–299 0.96 25
PcydA Cytochrome bd oxidase 350 318–386 0.96 18

The affinity of phosphorylated HrrA to the indicated regions was measured using purified protein in increasing concentrations and its ability to shift 10 nM DNA fragments of ∼100 bp size covering the maximal ChAP-Seq peak (for detailed information, see Supplementary Figure S9).