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. 2020 May 28;48(12):6740–6758. doi: 10.1093/nar/gkaa422

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Preparation of homogeneous tissue monolayers for analysis. (A) Human donor cornea in corneal viewing chamber with Optisol corneal storage media (Bausch & Lomb). (B) Corneal endothelium/Descemet's membrane monolayer being dissected from underlying stromal tissue. (C) Monolayer of corneal endothelial cells assumes a ‘scroll’ shape. This single cell monolayer will be used for sequencing and other analyses. (D) Immunostaining of monolayer of cells with corneal endothelial-specific marker, zonula occudens-1 (ZO-1) (Blue-DAPI).