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. 2020 May 28;48(12):6740–6758. doi: 10.1093/nar/gkaa422

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Intronic RNA is stabilized in Pre_S and FECD_REP tissue and FECD_REP patient-derived F35T & F45SV corneal endothelial cells. (A) Representative RNAseq data showing relative read number for TCF4 intron 2 RNA from each sample cohort. (B) Summary of RNA half-life of different cell lines after treatment with actinomycin D. The much longer half life of intron 2 up-stream RNA in F35T & F45SV cells is highlighted in red. (C) Graphs of time-dependent RNA decay following treatment with actinomycin D.