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. 2020 Jun 1;48(12):6583–6596. doi: 10.1093/nar/gkaa449

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figute 1. — NOL11 localises at the chromosome periphery. (A–C) Localisation of NOL11 at the PR. HeLa cells that stably expressed FLAG- and HA-tagged NOL11 (FLAG/HA-NOL11 HeLa cells) were fixed with 4% PFA after pre-extraction (B) or without pre-extraction (A) and stained with anti-HA antibodies (NOL11; green) and DAPI (blue). Representative cells in interphase (a), prometaphase (b), metaphase (c), and anaphase (d) are shown. Scale bar, 5 μm. (C) Chromosome spreads prepared from FLAG/HA-NOL11 HeLa cells were co-stained with anti-HA antibodies (NOL11; green) and ACA (red), anti-SMC2 antibodies (red), or anti-Ki67 antibodies (red) and then DAPI (blue). Scale bar, 2 μm. (D) Localisation of NOL11 in the chromosomal fraction of mitotic cells. HeLa cells were synchronised in mitosis and fractionated. Whole-cell extracts (Whole) and chromosomal fractions (Chr.) were immunoblotted with the indicated antibodies. HSP90 is a marker for cytosolic proteins and H3 is a marker for chromosomal proteins. Note that mobility of NOL11 in the chromosomal fraction appears slower than that in the Whole-cell extracts.