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. Author manuscript; available in PMC: 2020 Jul 6.
Published in final edited form as: Methods Mol Biol. 2015;1312:247–255. doi: 10.1007/978-1-4939-2694-7_26

Figure 2:

Figure 2:

Western blot transfer and immunoblotting of bovine Ro 60 and prestained molecular weight standards using 7, 10 and 12.5% gels (1.5 mm gels). Fig. 2A, 2B and 2C- Proteins on a 7, 10 and 12.5% SDS-PAGE gels transferred using heat. Lane 1-Anti-Ro 60 negative control; Lane 2- Anti-Ro 60 positive control; Lane 3-Conjugate control; Lane 4-Prestained molecular weight standards. (Reproduced from Ref 8 with permission from Elsevier).