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. Author manuscript; available in PMC: 2020 Jul 6.
Published in final edited form as: Methods Mol Biol. 2015;1312:247–255. doi: 10.1007/978-1-4939-2694-7_26

Figure 4:

Figure 4:

Immunoblots of purified Ro 60 and proteins derived from a HeLa cell extract transferred to nitrocellulose membrane using the heat transfer method (left) and by a conventional method (right).

Fig. 4A: Purified Ro 60 autoantigen immunoblot obtained from a 12.5% SDS-PAGE gel using the heat transfer method (left) and by a conventional method (right). Lane 1 – conjugate control; lanes 2 and 3 – normal controls; lane 4 – anti- Ro 60 SLE sera; lane 5 – prestained protein molecular weight standards.

Fig. 4B: HeLa cell extract immunoblot obtained from a 4-20 % gradient SDS-PAGE gel using the heat transfer method (left) and a conventional transfer method (right). Lane 1-conjugate control; lane 2 - normal control; lane 3-anti- Ro 60 SLE sera; lane 4 - anti-La sera; lane 5 - anti- Ro 52 sera; lane 6 - anti-Sm/nRNP sera; lane 7-prestained protein molecular weight markers (10 μL). (Reproduced from Ref 8 with permission from Elsevier).