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. Author manuscript; available in PMC: 2021 Sep 15.
Published in final edited form as: Carbohydr Polym. 2020 May 19;244:116443. doi: 10.1016/j.carbpol.2020.116443

Figure 5.

Figure 5.

GPC and NMR analysis of heparan sulfate substance from patient. A. GPC analysis, red, patient HS, green, HS standard from Celsus sourced from porcine intestine mucosa, blue, heparin standard, black, Enoxaparin. B. 1H NMR analysis of heparan sulfate from patient. The signals in porcine heparan sulfate were correspond to a. GlcN (Ac or S), H1 (5.3 ppm); b. IdoA, H1 (5.1 ppm); c. GlcA, H1 (4.4 ppm); d. IdoA2S, H2 (4.3 ppm); e. GlcNAc, H2 (3.8 ppm); f. GlcA, H4&H5 (3.6 ppm); g. GlcNS/GlcA, H3 (3.5 ppm); h. GlcA, H2 (3.3 ppm); i, GlcNS, H2 (3.2 ppm); j. N-acetyl (CH3) GlcNAc (2.0 ppm). The signals in heparin were correspond to A. GlcNS6S, H1 (5.5 ppm); B. GlcNS3S, H1 (5.4 ppm); C. GlcNY6X (where Y = Ac or S and X = H or S), H1 (5.3 ppm); D. IdoA2S, H1 (5.2 ppm); E. IdoA2SGlcNS, H1 (4.9 ppm); F. IdoA2S, H5 (4.7 ppm); G. GlcA, H1 (4.5 ppm); H. GlcNS, H6 (4.3 ppm); I. IdoA2S, H2 (4.3 ppm); J. GlcNS6S, H6 (4.2 ppm); K. IdoA2S, H3 (4.1 ppm); L. IdoA2S, H4 (4.0 ppm); M. GlcNS6S, H5 (3.9 ppm); N. GlcNS, H6 (3.8 ppm); O. GlcNS6S, H4 (3.7 ppm); P. GlcA, H3 (3.6 ppm); Q. GlcA/GlcNS3S, H2 (3.3 ppm); R. GlcNS, H2 (3.2 ppm); S. N-acetyl (CH3) GlcNAc (2.0 ppm).