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Migration of V. fischeri across TBS soft agar plates supplemented with (Left) 35 mM MgSO4 or (Middle) 10 mM CaCl2, or (Right) without supplement. The inner ring (black arrow) and outer ring (white arrow) can be readily seen for magnesium and calcium supplementation, and represent cells migrating to thymidine and serine, respectively. The rings take longer to form when cells are inoculated onto plates containing TBS without supplementation (Right).
