Table 1.
Antibiotic Concentrations and Medium for Selection of V. fischeri
| Antibiotic1,2 | Solvent | Stock Concentration (mg/mL) | Working Concentration (μg/mL) | Medium3 |
|---|---|---|---|---|
| Chloramphenicol | 100% EtOH | 1 | 1 | LBS |
| 5 | TMM or high copy # plasmid in LBS | |||
| Erythromycin | 100% EtOH | 2.5 | 2.5 | LBS |
| Gentamicin | ddH2O | 10 | 10 | LB |
| Kanamycin | ddH2O | 20 | 100 | LBS or TMM |
| Spectinomycin | ddH2O | 40 | 200 | LB |
| Tetracycline | 100% EtOH | 5 | 2.5 | LBS |
| 30 | SWT or TMM | |||
| Trimethoprim | 90% EtOH | 2 | 10 | LBS |
1
V. fischeri is highly resistant to ampicillin.
2
Zeocin has been used at a working concentration of 10 μg/mL in LB, but further optimization is necessary to enhance selectivity (Visick, Hodge-Hanson, Tischler, Bennett, & Mastrodomenico, 2018).
3
Optimal growth medium for different antibiotics was determined empirically.