| 75.3 mL | dH2O |
| 10 mL | 1 M HEPES, pH 7.5 or 1 M Tris, pH 7.5 |
| 6 mL | 5 M NaCl |
| 5 mL | 1 M MgSO4 heptahydrate |
| 100 μL | 33 mM K2HPO4 |
| 100 μL | 10 mM Ferrous Ammonium Sulfate |
| 500 μL | 20% NH4Cl |
| 1 mL | 20% D-glucose |
| 1 mL | 1 M KCl |
| 1 mL | 1 M CaCl2 dihydrate |
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