Table 1. PLK4 enzyme assays and substrates.
| Substrate sequence | Parental substrate sequence |
|---|---|
| Human PLK4 (bacteria) | Amino acids 1–269 |
| Human PTPN12 (wheat germ cell free) | Amino acids 1–780 |
| Human NMYC (wheat germ cell free) | Amino acids 1–464 |
| PLK4 CDK7/CAK substrate | 5FAM-FLAKSFGSPNRAYKK-CONH2 |
| PLK4 polybasic (‘RK’) substrate | 5FAM-RKKKSFYFKKHHH-CONH2 |
| NMYC substrate | 5FAM-KFELLPTPPLSPSR-CONH2 |
| PTPN12 substrate | 5FAM-TVSLTPSPTTQVET-CONH2 |
| CEP131 substrate 1 | 5FAM-NLRRSNSTTQVSQ-CONH2 |
| CEP131 substrate 2 | 5FAM-SQPRSGSPRPTEP-CONH2 |
Sequence of recombinant protein kinases and peptide substrates employed for assay of recombinant human PLK4. Sources of enzymes are included. 5FAM = 5-carboxyfluorescein.