Skip to main content
. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Curr Protoc Stem Cell Biol. 2020 Jun;53(1):e110. doi: 10.1002/cpsc.110

Table 1.

shows the components and concentrations in B8 media.

REAGENT CONCENTRATION
DMEM/F12
L-ascorbic acid 2-phosphate 200 μg/mL
Insulin 5 μg/mL
Transferrin 5 μg/mL
Sodium Selenite 20 ng/mL
Fibroblast Growth Factor 2-G3 (FGF2-G3) 40 ng/mL
Neuregulin 1 (NRG1) 0.1 ng/mL
Transforming Growth Factor Beta-3 (TGFß3) 0.1 ng/mL