Figure 1. Developmental, but not adult, adipocytes derive from a PDGFRα+ cell source.

(A–B) Pdgfra^Cre-ERT2; Rosa26R^RFP (PDGFRα-RFP) mice were administered tamoxifen (TM) (A) at postnatal day 10 (P10) and fed chow or HFD until P60 or (B) at P60 and fed chow or HFD until P120. IGW, PGW, and BATs were examined for direct RFP fluorescence either at (A) P60 or (B) P120 (chase). White arrowheads indicate the epididymis labeling. Scale = 100 μm. (C–D) RFP staining of IGW, PGW, and BATs from above P10-P60 and P60-P120 mice using immunohistochemistry (IHC). Scale = 200 μm.

Figure 1—figure supplement 1. Pdgfra-dependent RFP expression at pulse, P60-P180, and P60-P120 TZD pulse-chase.

(A–B) Pdgfra^Cre-ERT2; Rosa26R^RFP (PDGFRα-RFP) mice were administered TM (A) at postnatal day (P) 10 or (B) at P60. IGW, PGW, and BATs were examined for direct RFP fluorescence and RFP IHC staining after 3 days (pulse). Scale = 200 μm. (C) PDGFRα-RFP mice were administered TM at P60 and fed chow or HFD until P180. IGW, PGW, and BATs were examined for direct RFP fluorescence and RFP IHC staining at P180 (chase). Scale = 100 μm and 200 μm. (D) Quantification of RFP+ adipocytes observed in randomly chosen 10× magnification fields from IGW, PGW, and BAT sections. (E) Confocal immunofluorescence image of a representative IGW section from animals treated with TZD. Sections were stained with anti-RFP (red) and anti-perilipin (green) antibodies and counterstained with DAPI (blue; nuclei). Scale = 200 μm.