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. 2020 Jun 19;9:e56189. doi: 10.7554/eLife.56189

Figure 2. Developmental, but not adult, PDGFRα+ cells are adipogenic.

(A–B) PdgfraCre-ERT2; Rosa26RRFP (PDGFRα-RFP) male mice were administered TM at P10 (A) or P60 (B). Stromal vascular fraction of cells (SVF) were isolated from IGW of the mice after 3 days and cultured. The numbers indicate the percentage of the RFP+ labeled adipocytes. Scale = 100 μm. (C) TM-induced PDGFRα-RFP male mice either at P10 or P60 were fed chow or HFD until P120. SVF were isolated from IGW of the mice at P120, cultured, and examined for direct RFP fluorescence. Scale = 100 μm. (D) The real-time q-PCR analysis of adipogenic markers from cells described in C. *p<0.05 P10 RFP- compared to P10 RFP+ cells. (E) Gene expression levels of P10 and P60 RFP+ cells in SVF isolated from pooled IGW (n = 10). *p<0.05 P60 RFP+ compared to P10 RFP+ cells. Data are expressed as mean ± SEM. (F) PpargtTA-H2BGFP; PDGFRα-RFP male mice were administered TM at P10 or P60. SVF were isolated from the pooled IGW, PGW, and BAT depots (n = 8) after 3 days and sorted using RFP signal. GFP+RFP+ cells were quantified.

Figure 2.

Figure 2—figure supplement 1. Developmental, but not adult, PDGFRα+ cells overlap with PPARγ+ cells.

Figure 2—figure supplement 1.

(A) Schematic of PpargtTA-H2BGFP; PDGFRα-RFP male mice breeding. Mice were administered TM at P10 or P60. SVF were isolated after 3 days. GFP+RFP+ cells were quantified in IGW, PGW, and BAT. (B) The flow analysis of P10 labeled PDGFRα-RFP cells overlapping with PpargtTA labeled GFP+ cells. The numbers indicate the percentage of total SVF in IGW, PGW, and BAT (pooled SVF from n = 10 mice). (C) The flow analysis of P60 labeled PDGFRα-RFP cells overlapping with PpargtTA labeled GFP+ cells. The numbers indicate the percentage of total SVF in IGW, PGW, and BAT (pooled SVF from n = 8 mice).