Table 1. Primers used in this study.
| Purpose | Amplifies | Primers |
|---|---|---|
| PDR1 deletion | kanMX resistance cassette | 5’-CAGCCAAGAATATACAGAAAAGAATCCAAGAAACTGGAAGCGTACGCTGCAGGTCGAC-3’ 5’-GGAAGTTTTTGAGAACTTTTATCTATACAAACGTATACGTATCGATGAATTCGAGCTCG-3’ |
| PDR3 deletion | Nourseothricin resistance cassette | 5’-ATCAGCAGTTTTATTAATTTTTTCTTATTGCGTGACCGCACGTACGCTGCAGGTCGAC-3’ 5’-TACTATGGTTATGCTCTGCTTCCCTATTTTCTTTGCGTTTATCGATGAATTCGAGCTCG-3’ |
| GGA1 deletion | Hygromycin resistance cassette | 5’-AGTCACTACTTCAAGTATAACCCAGACAAGAGTCTTTTAAATAGCTTGCCTTGTCCCCGC-3’ 5’-ATGGCATCTACTTTTTTTTCAACTTCTCTACCGAATTTGACGTTTTCGACACTGGATGGC-3’ |
| VPS10 deletion | VPS10 5’ upstream | 5’-CCCAAACTAAAAAGTATCCGCCTGT-3’ 5’-GACAAGGCAAGCTAACGTGTGATGACTACTGGACACT-3’ |
| VPS10 3’ downstream | 5’-GCCATCCAGTGTCGAAGAGATTACTTTACATAGAGTAGATAATTCCATATACTTTTCATA −3’ 5’-AATGAAGTACTATAAATATTAAAGTACGTTAGTAGTTTATTTCTCTTCGG-3’ |
|
| Hygromycin resistance cassette | 5’-TCATCACACGTTAGCTTGCCTTGTCCCCGC-3’ 5’-TGTAAAGTAATCTCTTCGACACTGGATGGCGG-3’ |
|
| APM3 deletion | APM3 5’ upstream | 5’-AGGGGTAGAAGTCGCTGATTGAT-3’ 5’-GGGCCTCCATGTCCTATTTTGGTTGGGTTGGTAAGGTTTACAG-3’ |
| APM3 3’ downstream | 5’-GCTGGTCGCTATACTGTTATATGTGTACTTGAAATTCCATGCGAAACTAAA-3’ 5’-TGCGGAAGTCTTCCCTAAGACG-3’ |
|
| Hygromycin resistance cassette | 5’-CAACCAAAATAGGACATGGAGGCCCAGAATACCC-3’ 5’-TCAAGTACACATATAACAGTATAGCGACCAGCATTCACA-3’ |
|
| APL4 deletion | APL4 5’ upstream | 5’-ATGTATATAATTCCGGAAGTGTGGTCCT-3’ 5’-GACAAGGCAAGCTTATGGTGTTCAGGTCTTTCTCGTTGCT-3’ |
| APL4 3’ downstream | 5’-CCATCCAGTGTCGAAAAATGCCTTTAAAATTACAGAACATAACATGATTAATGAC-3’ 5’-GAATTCTGGTCCAAGGCAATTCTATATTTGAT-3’ |
|
| Hygromycin resistance cassette | 5’-CCTGAACACCATAAGCTTGCCTTGTCCCCG-3’ 5’-TTTTAAAGGCATTTTTCGACACTGGATGGCGG-3’ |
|
| GGA2 deletion | GGA2 5’ upstream | 5’-GATTTCTACAGTCTTTCTGATGGGTTCTTGG-3’ 5’-ACGATATTCTTAGACATGATGCAGTATCACGATTAGCAAT-3’ |
| GGA2 3’ downstream | 5’-AATCTTGGCTTAATCCTCTGGCGTTTCTTATCAATCCTTTCT-3’ 5’-TCTTCCTTTGAAGAAAATTCGTCCTCATCT-3’ |
|
| K. lactis LEU2 | 5’-AATCGTGATACTGCATCATGTCTAAGAATATCGTTGTCCTACCGG-3’ 5’-GAAACGCCAGAGGATTAAGCCAAGATTTCCTTGACAGCC-3’ |
|
| Integration at TRP1 | TRP1 locus | 5’-GTGTACTTTGCAGTTATGACG-3’ 5’-AGTCAACCCCCTGCGATGTATATTTTCCTG-3’ |