Fig. 5.

Schematics for (A) staggered DIA windows, where (B) common peaks in the previous and next scan cycles can be used to computationally cut the precursor isolation window in half. Here the Time = T₀ MS/MS scan acquired with a 600–624 m/z precursor isolation window is used as an example. Green ions, which correspond to peaks that can be found in the 588–612 m/z windows in the previous and next scans, can only come from peptides between 600–612 m/z. Similarly, blue ions can only come from peptides between 612–624 m/z. Red ions are found in both sets of previous and next scans and are fractionally allocated to both computationally demultiplexed MS/MS, whereas orange ions are not found in any neighboring scans and can be considered noise.