Fig. 5.

Subcellular location of source proteins and the relationship between deamidation of peptides containing the NX(S/T) motif. A, A high proportion of deamidated HLA-I bound peptides contain a consensus N-linked glycosylation motif which is not reflected in peptides derived from tryptic/elastase digestion of cellular material. B, A comparison of the subcellular location of the source antigens for HLA-I bound peptides among unmodified and peptides with native or deamidated Asparagine residues that harbor the NX(S/T) glycosylation motif. C, A comparison of the subcellular location of the source proteins of deamidated peptides bearing the NX(S/T) motif shows significant differences between tryptic/elastase-digested proteomes and HLA-I bound peptides. Significance was determined by two-way analysis of variance (ANOVA) performed using Prism 7 (***, p = 0.0002, **, p = 0.0021).