Fig. 7.
The supercomplex in HIGD2AKO cells lacks COX3. A, The indicated cell lines were analyzed by BN and SDS-PAGE and immunoblotting with the indicated antibodies. B, Affinity-enrichment mass spectrometry (AE-MS) was performed on the NDUFV3FLAG and control cell lines and significant FLAG interaction partners visualized through a volcano plot. The curved line indicates significance determined through an FDR-based approach (FDR<5%, S0 = 1). n = 3 independent subcultures. C, as for B however, the enrichment was performed on NDUFV3FLAG/HIGD2AKO relative to the NDUFV3FLAG cell line.