a. Overview of two genes that exhibit broad immunophenotypes. Colours as in Fig 4. Statistical methods and sample size differ between parameters – see Materials and Methods and www.immunophenotype.org for specific gene/parameter combination of n.
b. Phenotypic abnormalities PBL in Duoxa2–/– mice. Relative PBL cell subset frequencies for Duoxa2–/– (n=11) versus wt mice (n>450 per sex for all parameters). Dark and light blue denote wt males and females; red and orange denote Duoxa2–/– males and females.
c. Effector cell subsets in Bach2–/– mice. Fold-change in SPL cell subset composition in mutant mice (n=6) versus wt controls (n=76). Yellow represents significant cellular phenotypes not previously reported, as detected by reference range; dark blue represents significant cellular phenotypes previously reported; light blue represents non-significant differences. KLRG1+CD4- NK cells: 10-fold increase, p=1.1x10-9; reference range combined with Fisher’s exact test).
d. Cytotoxic T lymphocytes in Bach2–/– mice (n=4) compared to wt controls (212 female and 208 male): grey points represent individual wt mice; red circles represent individual Bach2–/– mice.
e. DSS colitis in Bach2–/– mice (n=4) compared to wt controls (n=481 female and n=315male): grey points represent individual wt mice; white circles represent wt mean values; blue circles represent individual Bach2–/– mice.