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. 2020 Jul 3;9(6):bio054171. doi: 10.1242/bio.054171

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© 2020. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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graphic file with name biolopen-9-054171-g1.jpg — Pry3 accumulates in the cell wall next to bud scars, and is absent from mating projection. This highly polarized distribution is abolished when Pry3 is over-expressed. Depicted here are Pry3-mCherry and calcofluor-white (CFW) signal.